The main goal was to investigate the existence of differences in motor abilities between two groups, experimental and control. Children who participated in testing the motor skills are participants of the Kindergarten Maslačak in Garešnica. A total of 35 preschool children, who were also the experimental group, participated in the research. The testing was compared with a control group consisting of a group of 34 children of the same older age from Zagreb. Experimental testing was performed in 2022, while testing of the control group was performed in 2017. In order to get the results of morphological characteristics, three components were used: body height, weight and body mass index. For further analysis of motor and functional abilities, seven components were used and the final average value is calculated based on them. The tests that were carried out were: standing with one leg on a cube, backward polygon, sit-ups in 30 seconds, jumping into a further place, hand tapping for 10 seconds, trunk bending and running on a 60-meter long track in a time period of 90 seconds. The obtained test results are presented descriptively, and analysis of variance (ANOVA) was used for statistical difference results. The collected results confirm that there is a statistically significant difference in the MSDM, MRSJNK, MFSR, MBTR and MST30 tests, and based on them, it can be concluded that the hypothesis is accepted. Thus, the experimental and control groups differ in their motor skills because the children practice more and such a program has a stimulating effect on their motor development.